Colocalization features

Description

Colocalization features quantify the degree to which two different spectral channels spatially co-occur within a segmented object. these measurements assess the relationship and correlation between different protein markers.

Overview

Colocalization features are computed for each unique pair of channels within the same segmented object.

Features extracted

Correlation-based metrics

Feature	description
MEAN.CORRELATION.COEFF	mean pearson correlation coefficient
MEDIAN.CORRELATION.COEFF	median pearson correlation coefficient
MIN.CORRELATION.COEFF	minimum correlation coefficient
MAX.CORRELATION.COEFF	maximum correlation coefficient

Manders coefficients (M1 and M2)

Feature	description
MEAN.MANDERS.COEFF.M1	average fraction of channel 1 overlapping with channel 2
MEDIAN.MANDERS.COEFF.M1	median M1 coefficient
MIN/MAX.MANDERS.COEFF.M1	min/max M1 values
MEAN.MANDERS.COEFF.M2	average fraction of channel 2 overlapping with channel 1
MEDIAN.MANDERS.COEFF.M2	median M2 coefficient
MIN/MAX.MANDERS.COEFF.M2	min/max M2 values

Overlap coefficient

Feature	description
MEAN.OVERLAP.COEFF	average overlap coefficient
MEDIAN.OVERLAP.COEFF	median overlap coefficient
MIN/MAX.OVERLAP.COEFF	min/max overlap coefficients

K1 and K2 coefficients

Feature	description
MEAN.K1	average intensity correlation quotient for channel 1
MEDIAN.K1	median K1 coefficient
MIN/MAX.K1	min/max K1 values
MEAN.K2	average intensity correlation quotient for channel 2
MEDIAN.K2	median K2 coefficient
MIN/MAX.K2	min/max K2 values

Use cases

Colocalization features are useful for:

Determining protein-protein interactions
Assessing subcellular localization patterns
Identifying co-distributed cellular components
Quantifying marker overlap in immunofluorescence