Intensity features
Description
Intensity features quantify the pixel/voxel value distributions within segmented objects. these measurements capture both overall intensity levels and spatial intensity patterns.
Features extracted
Location-based intensity
Feature |
description |
|---|---|
CM.X / CM.Y / CM.Z |
average intensity-weighted coordinates in each spatial dimension |
CMI.X / CMI.Y / CMI.Z |
center of mass intensity in each spatial dimension |
I.X / I.Y / I.Z |
integrated intensity along each axis |
MAX.X / MAX.Y / MAX.Z |
coordinates of maximum intensity |
Statistical measures
Feature |
description |
|---|---|
MEAN.INTENSITY |
average intensity within object |
MEDIAN.INTENSITY |
median intensity value |
MAX.INTENSITY |
maximum intensity value |
MIN.INTENSITY |
minimum intensity value |
STD.INTENSITY |
standard deviation of intensity |
MAD.INTENSITY |
median absolute deviation |
LOWER.QUARTILE.INTENSITY |
25th percentile |
UPPER.QUARTILE.INTENSITY |
75th percentile |
Edge-based measurements
Feature |
description |
|---|---|
INTEGRATED.INTENSITY.EDGE |
sum of edge pixel intensities |
MEAN.INTENSITY.EDGE |
average edge intensity |
MAX.INTENSITY.EDGE |
maximum edge intensity |
MIN.INTENSITY.EDGE |
minimum edge intensity |
STD.INTENSITY.EDGE |
standard deviation of edge intensities |
EDGE.COUNT |
number of edge voxels |
Other measurements
Feature |
description |
|---|---|
VOLUME |
object volume (included for reference) |
DIFF.X / DIFF.Y / DIFF.Z |
spatial intensity gradient in each dimension |
MASS.DISPLACEMENT |
distance between geometric and intensity centers |
Calculation method
Intensity features are computed as follows:
Segmentation masking: apply object masks to isolate pixel/voxel values
Statistical computation: calculate mean, median, max, min, std, etc.
Spatial analysis: determine intensity-weighted coordinates and gradients
Edge detection: identify edge pixels/voxels and compute edge statistics
Applications
Intensity features are useful for:
Quantifying fluorescence levels within cellular structures
Detecting changes in protein expression
Characterizing subcellular localization patterns
Identifying phenotypic alterations due to treatments